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AF488 azide | CAS 1679326-36-3 (with azidohexyl group)
| Catalog Number | R01-0044 |
| Category | Alexa Fluor |
| Molecular Formula | C36H56N8O10S2 |
| Molecular Weight | 825.01 |
| Catalog Number | Size | Price | Quantity |
|---|---|---|---|
| R01-0044 | -- | -- |
* Please be kindly noted products are not for therapeutic use. We do not sell to patients.
Product Introduction
AF488 is a fluorescent dye. AF488 is a fluorophore pH-insensitive over a broad pH range (from 4 to 10). It has an absorption maximum at 495 nm and an emission maximum at 519 nm in the green spectrum region. The dye is hydrophilic and can be used to introduce the fluorescent label into various molecules, including proteins and antibodies. Conjugates of molecules with AF488 have high brightness and photostability and are commonly used in flow cytometry and microscopy. This allows the detection of biological objects with high sensitivity at a longer imaging time.,AF488 azide interacts with alkynyl derivatives of biomolecules in Click Chemistry reactions either in the presence of copper (I) catalyst (with terminal alkynes) or without catalyst (with cyclooctynes), leading to the formation of stable adducts.
Chemical Information
Product Specification
| Purity | NMR 1H, HPLC-MS (95%) |
| Solubility | Good in water, DMF, DMSO |
| Appearance | Orange solid |
| CF260 | 0.16 |
| CF280 | 0.10 |
| Fluorescence Quantum Yield | 0.91 |
| Mass Spec M+ Increment | 616.1 |
| Excitation | 495 |
| Emission | 519 |
| Storage | Storage: 24 months after receival at -20 °C in the Dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. |
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